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https://avalanches.com/pk/abbottabad_umair_masood1757313_22_06_2021


DNA synthesis by using Glycosyltransferases Micro Fluid Chip

Umair Masood


DNA synthesis is a molecular biology process in which the DNA is created by using artificial method. There is different standard method for gene synthesis like: oligonucleotide synthesis, endonuclease-mediated assembly, annealing base connection of oligonucleotide. Oligonucleotide synthesis is methods of relatively short fragment of nucleotide with define sequence. The production of oligonucleotide the necessary bases adenine, thymine, guanine, and cytosine is added the synthesis is a computer control process that repeats several steps until full length product. Finally, the concentration of each oligonucleotide adjusted. The mixture of oligonucleotide is than provided gene synthesis lab. Oligo lab is assembled into the gene of your choice assembly by PCR (Polymerase chain reaction). DNA is made of nucleotides each nucleotide contains a phosphate group, a sugar (deoxyribose) and four nitrogenous bases adenine, guanine, cytosine, thymine. A deoxyribose sugar is the five carbon sugar molecules that help form the phosphate backbone of DNA. Deoxyribose sugar is a lacking of one oxygen atom that why called deoxyribose sugar. A deoxyribose sugar contains a “OH” group which play important role in our technique. Gene synthesis by using Glycosyltransferases Micro Fluid Chip method is simple and cost-effective method which DNA is synthesis by using syringe nozzle and micro fluid chip. Syringe consists of four types of nozzle and four types of syringes each nozzle is fill with different nucleotides (A,T, C,G). Nozzle drop it nucleotides drops on the micro fluid chip according to the sequence. The micro fluid chip carries short drops of nucleotides and Glycosyltransferases start adds DNA nucleotide according to the arrangement of drops or nucleotide.


Link:

https://www.academia.edu/49340552/DNA_synthesis_by_using_Glycosyltransferases_Micro_Fluid_Chip

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https://avalanches.com/pk/abbottabad__meet_the_22yearold_muhammad_sohaib_from_mansehra_whos_transforming1513367_09_04_2021

Meet The 22-Year-Old Muhammad Sohaib From Mansehra Who’s Transforming People With His Fitness Trainings

The craze for fitness in Pakistan is on a roll and it’s amazing to see how more and more people are eagerly flocking towards a better lifestyle; prioritizing their health. A healthy culture is now taking shape and it is easily a sign of a progressive society. But things are changing and so is everyone’s mindsets said by Muhammad Sohaib.

We know that life is all about finding the right motivation to do something. For those who know what struggle is, they know what it takes to build yourself from scratch into something that can give motivation to others. Majority of the times, these are the same people who take the wheel of life in their own hands and write their own stories. And just like this, we came across Sohaib !

Meet the 22-year-old fitness trainer, Muhammad Sohaib , who’s based in Mansehra the first Pakistani young fitness trainer who transformed many people by giving them fitness training and diet


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https://avalanches.com/pk/abbottabad_meet_the_22yearold_muhammad_sohaib_from_mansehra_whos_transforming_1497224_04_04_2021
https://avalanches.com/pk/abbottabad_meet_the_22yearold_muhammad_sohaib_from_mansehra_whos_transforming_1497224_04_04_2021

The craze for fitness in Pakistan is on a roll and it’s amazing to see how more and more people are eagerly flocking towards a better lifestyle; prioritizing their health. A healthy culture is now taking shape and it is easily a sign of a progressive society. But things are changing and so is everyone’s mindsets said by Muhammad Sohaib.

We know that life is all about finding the right motivation to do something. For those who know what struggle is, they know what it takes to build yourself from scratch into something that can give motivation to others. Majority of the times, these are the same people who take the wheel of life in their own hands and write their own stories. And just like this, we came across Sohaib

Meet the 22-year-old fitness trainer, Muhammad Sohaib , who’s based in Mansehra the first Pakistani young fitness trainer who transformed many people by giving them fitness training and diet


With years long of information in the fitness industry and a vast list of elite clients, Sohaib lets help others achieve their fitness goals! But what’s even more interesting is that Muhammad Sohaib fitness trainer is not just a fitness trainer but also an nutritionist.

So, if you’re inspired and motivated and really want to know more about this And if you’re looking for more of his amazing transformations and work, you can check out his Instagram account @muhammadsohaib_officialwhere he keeps his fans updated on everything or simply check out his YouTube click here

To conclude it all, it’s always better to speak to a fitness trainer before you opt for losing weight or working out. To avoid any mishaps, these fitness trainers have immense knowledge of the human body and how one should go about transforming themselves. So, before you make the crazy decision of heading to the gym and getting busy with heavy workout routines, consultation is important. Also, always remember to prioritize your health because there’s nothing more precious than it!


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https://avalanches.com/pk/abbottbd_hiv_structure_degradation_and_hiv_cure1207621_10_01_2021

HIV structure degradation and HIV cure:

Umair Masood:

When HIV first enters the human body or human blood stream the virus start circulates throughout the body. The protective surface of HIV is study with particular protein called gp120 and lip membrane envelop inside the capside that contain a viral RNA and viral enzyme.Gp120 protein on the virus is only able to bind with a cell surface called CD4-receptor.The second receptor protein ccr5 present on the surface of macrophages for the virus enter the cell by endocystosis and HIV cycle begin. From given Diagram virus Receptor attached with Lipid envelops if we can break the structure of Lipid envelops by using a enzyme called lipase virus could not be able to attach the surface of cell because the structure of the virus is degrade. The special type of lipase called pancreatic lipase which is working in 37°C and 7.0 PH can be used in this process


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https://avalanches.com/pk/abbottbd_dna_fingerprinting_and_crispr_cas9_system1204381_09_01_2021

DNA fingerprinting and CRISPR cas9 system:

Umair Masood

Leicester university geneticist Alec Jeffreys develops a technique called DNA fingerprinting in 1985 it allows the DNA sample from different people to be compared look for similarities and differences. It used the solving crime and can confirm if the people are related to each other like paternity testing. There is a section of chromosomes where instead of a gene consisting of a long sequence of bases, they are much shorter sequencing of three to five base that is repeated in many times these repeated sequences called short tandem repeats. If we can know the STR sequence of any person then we can design a gRNA.CRISPR to scan through DNA or find specific STR sequences of another person. If the CRISPR does not find the targeted STR sequence it does not bind to it its means that no fluorescence color appears under UV-light but its scan and find its target and this binding create a fluorescence signal its means that STR can occur in given DNA and both persons are related to each other with respect to any point that is father or son or suspect and victim. The DNA we can check is must be labeled with fluorescence color.


Reference:

https://www.academia.edu/44639177/DNA_fingerprinting_and_CRISPR_cas9_system

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https://avalanches.com/pk/abbottbd_muhammad_sohaib_fitness_trainer_1191491_05_01_2021

Muhammad Sohaib fitness trainer

Muhammad Sohaib is a fitness trainer and fitness model who helped in body transformation of many people.

he said that staying active is one of the best ways to keep our bodies healthy. But did you know it can also improve your overall well-being and quality of life?

It’s a natural mood lifter.

Regular physical activity can relieve stress, anxiety, depression and anger. You know that "feel good sensation" you get after doing something physical? Think of it as a happy pill with no side effects! Most people notice they feel better over time as physical activity becomes a regular part of their lives.

It keeps you physically fit and able.

Without regular activity, your body slowly loses its strength, stamina and ability to function properly. It’s like the old saying: you don’t stop moving from growing old, you grow old from stopping moving. Exercise increases muscle strength, which in turn increases your ability to do other physical activities.


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https://avalanches.com/pk/abbottbd__mutation_detection_via_crisprcas9_microfluid_chip_1081754_04_12_2020

Mutation detection via CRISPRCas9 micro-fluid chip:

Author: Umair Masood


In biology mutation is a change in the nucleotide sequences of the DNA of an organism Mainly there are three types of mutation: point mutation, deletion and insertions. Once the mutation has been defined allele specific oligonucleotide hybridization, amplification, heteroduplex formation method referred to as a diagnostic method some advance technique like CRISPR cas9 system is using for selected mutagenesis. Using microfluid and CRISPR cas9 system we can detect a mutation. Let's say you have a DNA sample with fluorescent labeled from patient and you want to make sure that gene you are interested is in healthy gene. We can design a CRISPR to scan through DNA or find specific gene or mutation. The CRISPR scan the DNA if the CRISPR does not find targeted gene it does not bind to it its means that no fluorescence color appears under UV-light but its scan and find its target and this binding create a fluorescence signal its means that mutation can be occur in gene


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https://avalanches.com/pk/abbottbd__identify_a_gene_that_makes_protein_by_using_cell_free_protein_synthes1074213_02_12_2020

Identify a gene that makes protein by using cell free protein synthesis system:


Author: Umair Masood


Living cell could genetically modify to perform a faction such as production of protein. However, these genetically modification often dispute with normal cellular function and result in mutation. Defect can be overcome through removing the bacterial membrane which leave the lysate that is perform both transcription and translation. The cell free-protein synthesis also known as invitro protein synthesis is the production of protein without uses of living cell. Gene is act as instruction to make protein. If we can isolate a gene and then apply a cell free protein synthesis system after synthesis the protein, run on gel-electrophoresis we can identify a gene on the basis of protein. Gel electrophoresis is a laboratory technique used to contrasting proteins according to molecular size and charge.

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https://avalanches.com/pk/abbottbd__protein_sequence_via_nanopore_sequencing_machine_1071044_01_12_2020

Protein sequence via nanopore sequencing machine:

Author: Umair Masood:

Protein is a biomolecule consist of one or more chain of amino acid residues. Protein have significance role in the structure function organization of the cell. Linear amino acid residues are called a polypeptide. Protein contain short and long amino acid polypeptide less than 20-30 residues. The building block of protein is amino acid which are small organic molecules consist of alpha carbon atom link to an amino group and carboxyl group hydrogen atom are variable components called side chain. All protein has primary secondary tertiary and quaternary structur.in the protein sequencing the major steps is first secondary tertiary and quaternary structure convert into the primer structure which is simpler as compare to other than primary structure is load into the nanopore sequencing machine. Nanopore sequencing machine work by monitoring changes to an electrical current as a nucleic acid are passed in a protein Nanopore, then signal is decoded and provide a particular DNA or RNA sequence our case nanopore sequencing machine work by monitoring change and electrical current as an amino acid which are passing through nanopore protein channel then signal is decoded and provide a particular amino acid sequence.



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https://avalanches.com/pk/abbottbd_surma_kaala_song_is_out_now_in_our_channel_like_share_and_subscribe_li1043529_23_11_2020

SURMA KAALA SONG IS out now in our channel like share and subscribe link in below:

https://www.dailymotion.com/video/x7b85ld?fbclid=IwAR1Xu4E_E2Hgu07Ucz5bmvtwDQNbgvK1BnGK7iZTaxbzni7JUA_lZcWWaQU

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https://avalanches.com/pk/abbottbd__invitro_site_specific_dna_editing_via_restriction_enzyme_1040724_22_11_2020

in-Vitro Site specific DNA Editing Via Restriction Enzyme:


Author: Umair Masood

full paper: https://www.academia.edu/44551733/In_Vitro_Site_specific_DNA_Editing_Via_Restriction_Enzyme

A few years ago, some researchers realized that they could use CRISPR cas9 to gene-editing tools. A part of DNA I like to change if I know the sequence of a gene. I can build a CRISPR that carry a matching code. one inside the cell CRISPR cas9 will scan the DNA until it finds that exact spots. when it does, it cut the DNA right there. now I have the broken gene I can inserted new sequence into the gap. Restriction digestion is a process in which DNA is cut at specific sites. Restriction enzyme bind and cleave double stranded DNA at specific site that main property of restriction enzyme used in DNA editing. Basically, the whole idea is depending upon two things: DNA denaturation and short fragment of DNA is bind to the template DNA and restriction enzyme cleave only double stranded DNA at specific site.

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https://avalanches.com/pk/abbottbd_announcement_for_kid_watch_motu_patlu_i_our_channel_like_share_and_com1036996_21_11_2020

Announcement for kid watch motu patlu i our channel like share and comment link in below


https://www.dailymotion.com/video/x7xlm3q?fbclid=IwAR0eGq1eRLFmo-ge8Zz38-6IZk0EdtHTExlD3Win2nfcBsDv3hI90KUu1ws

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https://avalanches.com/pk/abbottbd_daddy_mummyy_song_out_now_enjoy_like_share_and_subscribe_link_in_bel1033529_20_11_2020

"DADDY MUMMYY" song out now enjoy like share and subscribe link in below


https://www.dailymotion.com/video/x54savy?fbclid=IwAR0Bphf8o23tfdbTjwsDpCTzj7yQynukwqq7ntQ8Ufkm7W81MrbNSavQWas

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https://avalanches.com/pk/abbottbd_coka_our_new_song_iss_out_now_like_share_and_subscribe_link_in_below1033528_20_11_2020

"COKA" OUR NEW SONG ISS OUT NOW LIKE SHARE AND SUBSCRIBE LINK IN BELOW:

https://l.facebook.com/l.php?u=https%3A%2F%2Fdai.ly%2Fx7ap1cy%3Ffbclid%3DIwAR0Bphf8o23tfdbTjwsDpCTzj7yQynukwqq7ntQ8Ufkm7W81MrbNSavQWas&h=AT3htNP6sxRIx1aMgNMM396HINVjKziDIiSEqpHqKONW8UIq_LkPxAu9SYjrzEbURJr1PKfDsKRuSqyVYcdwzlEQ3J_NeMxCcl2utFU-kpc0bl7NQUtD4KN_AWzx_qkEgSk&__tn__=-UK-R&c[0]=AT1YdXeG4PVJq5x8eYNaN9mr3C8XEg2s3-WtB-Nth_fOT_x1cJmv3aDaA6QVxKdqag7g8BOR0x3RBuHpG_n24SHNNbkDyZvxMhjFsPZTOoS7QmV3WUmnG24Q3EfCbeaeHbr8pk3pEnF2kcraBdOikHGb

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https://avalanches.com/pk/abbottbd_build_muscles_by_muhammad_sohaib_1032420_19_11_2020


We know that being active is a great way to keep our body healthy. But do you know that it can also

improve your overall well-being and quality of life

I started physical activities workout 2 years ago first 2 month i don’t get any results but i never give up

and after 7 month my body physique was so good i feel better with that body and feel energetic healthy.

I will let you know how you can gain weight loose weight or to be fit.

Muhammad Sohaib fitness

How to gain weight and muscles :

Don’t drink water before meals. This can fill your stomach and make it harder to get in enough calories.

Eat more often. …

Drink milk. …

Try weight gainer shakes. …

Use bigger plates. …

Add cream to your coffee. …

Get quality sleep.

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https://avalanches.com/pk/mardan_waqar_khan_is_a_pakistani_model_actor_singer_waqar_khan_born_in_304453453_24_09_2022

Waqar Khan is a Pakistani Model, Actor, Singer. Waqar Khan born in 30, June 1992 in Mardan Pakistan,

Waqar khan family In Mardan Pakistan. his father Saif Ullah khan is a businessman.

Waqar khan Belong to Pukhtoon family. Waqar completed his graduation in Awkum.

Waqar first drama on Avt Khyber drama name Mor baibay ao naway EP # 09.

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