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Sixty-six million years ago, an asteroid hit the Earth with the force of 10 billion atomic bombs and changed the course of evolution. The skies darkened and plants stopped photosynthesising. The plants died, then the animals that fed on them. The food chain collapsed. Over 90% of all species vanished. When the dust settled, all dinosaurs except a handful of birds had gone extinct.
Cassette recruitment (attC × attI recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (KmR) suicide vector pJP5603 with an attC site is delivered into the recipient E. coli UB5201 strain via conjugation. The recipient strain carries an intI1 gene, expressed from the inducible PBAD promoter, and an attI1 site, residing on the carbenicillin resistance (CbR) pBAD24 and chloramphenicol resistance (CmR) pACYC184 backbones, respectively. The donor suicide vector cannot replicate within the recipient host and thus can only persist following attC × attI recombination to form a plasmid cointegrate. (B) Average recombination frequencies (log10 scale, ±1 SE) between attI1 and nine archaeal attCs (with phyla of origin labeled along the x axis) and the paradigmatic bacterial attC site (attCaadA7), used as positive control. Average frequencies were calculated following three independent cassette insertion assays (see Materials and Methods for details). No statistically significant difference in recombination frequencies were detected among the tested attCs (Kruskal-Wallis test, n = 27; df = 8, P = 0.488). Recombination frequencies are shown for attC bottom strands only. See table S1 for attC top strand recombination frequencies. N.S., not significant. Credit: Science Advances (2022). DOI: 10.1126/sciadv.abq6376
A team of researchers at Macquarie University, in Australia, has found evidence showing that some Archaea have integrons. In their paper published in the journal Science Advances, the group describes how they used a recently developed technique called metagenome-assembled genomes (MAG) to study the genomes of Archaea samples in a new way, and what they learned by doing so.
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The European Service Module is powering Orion to the Moon and back, providing electricity, propulsion, keeping electronics and the crew module at the right temperature. On the next Artemis missions, it will also provide water and air for astronauts.Artemis I is an uncrewed mission that is being used to demonstrate and test the Orion spacecrafts capabilities. It also affords mission control the opportunity to get used to flying Orion and operating the European Service Module.Yesterdays firing of the engines has pushed Orion in the opposite direction of travel to our Moon, and occurred relatively far away from the lunar surface, putting the spacecraft into a so-called distant retrograde orbit. This elongated orbit around the Moon uses little fuel. Orions mission profile will see it leave halfway before the full twelve-day orbit, firing its thrusters in six days to fly closer to the Moon on a final flyby and then slingshot back to Earth.
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Ministerul Investiiilor i Proiectelor Europene a anunat, astzi, c va oferi, în 2023, românilor vulnerabili, din fonduri europene, sprijin pentru plata facturilor la energie în valoare de 1.400 de lei.Potrivit unui comunicat al MIPE, sprijinul acordat populaiei vulnerabile pentru compensarea preurilor la energie va fi în valoare nominal de 1.400 lei, poate fi utilizat pân la data de 31 decembrie 2023 i va fi acordat pe loc de consum/gospodrie vulnerabil, în dou trane, astfel: